: The protein sample is aspirated through the tip, binding to the C4 resin.
This technology has been instrumental in recent studies, including identifying SARS-CoV-2 protease cleavage sites and characterizing human cannabinoid receptors. c4.zip
A C4 ZipTip is a standard pipette tip containing a microscopic bed of resin fixed at its distal end. Unlike C18 resins, which are used for smaller peptides, the C4 resin is optimized for larger, more hydrophobic molecules like proteins and large polypeptides. Primary Uses in Research Researchers use these tools for two main reasons: : The protein sample is aspirated through the
: Contaminants are washed away with a dilute acid, such as trifluoroacetic acid (TFA) . Unlike C18 resins, which are used for smaller
: Best for proteins > 10 kDa and highly hydrophobic peptides.
Expression, Purification and Characterization of the Human ... - Nature