Rbd-991 -

: The target fragment (bases 991–1749) corresponding to amino acids 331–583 is synthesized to represent the Receptor-Binding Domain (RBD). Vector Digestion : An expression vector, such as pQE30

: The synthesized fragment is ligated into the vector using kits like the ClonExpress® Ultra One Step Cloning Kit and then transformed into a host like E. coli XL1-Blue . RBD-991

Depending on the field, "preparing a piece" for a 991 might mean: : The target fragment (bases 991–1749) corresponding to